Brian P. Weiser, Ph.D.

Associate Professor

Science Center 307A
Phone: 856 566-6270; Fax: 856 566-6291
weiser@rowan.edu 

Education

Johns Hopkins University, Baltimore, MD
Postdoc (Pharmacology / Enzymology)

University of Pennsylvania, Philadelphia, PA
Ph.D. (Pharmacology)

Albright College, Reading, PA
B.S. (Biology)

 

Research Interests

The Weiser Lab has several interests: (1) Understanding how protein-protein interactions influence protein activity; (2) Discovering small molecules that can be used therapeutically or to explore protein function; (3) Developing statistical methods to understand non-canonical dose-response relationships.

Our projects examine DNA repair proteins, sirtuin deacylases, and various macromolecular complexes. We use traditional biochemistry methods along with computational, structural, and statistical modeling.

Keywords: replication protein A (RPA), uracil DNA glycosylase (UNG2), sirtuin isoform 2 (SIRT2), proliferating cell nuclear antigen (PCNA), propofol, fluorescence, hormesis, 1-aminoanthracene, dose-response

We welcome all opportunities to collaborate with other research groups to share our technical and material resources!

Visit Weiser's full publication list

 

Selected Publications 

(1) Yang J, Cassel J, Boyle BC, Oppong D, Ahn YH, Weiser BP (2024) A homogeneous time-resolved fluorescence screen to identify SIRT2 deacetylase and defatty-acylase inhibitors. PLOS ONE 19: e0305000.

(2) Yang J, Nicely NI, Weiser BP (2023) Effects of dimerization on the deacylase activities of human SIRT2. Biochemistry 62: 3383-3395.

(3) Abbaraju VD, Robinson TL, Weiser BP (2023) Modeling biphasic, non-sigmoidal dose-response relationships: comparison of Brain-Cousens and Cedergreen models for a biochemical dataset. ArXiv doi.org/10.48550/arXiv.2308.08618.

(4) Greenwood SN, Kulkarni RS, Mikhail M, Weiser BP (2023) Replication protein A enhances kinetics of uracil DNA glycosylase on ssDNA and across DNA junctions: explored with a DNA repair complex produced with SpyCatcher/SpyTag ligation. ChemBioChem 24: e202200765.

(5) Hong JY, Cassel J, Yang J, Lin H, Weiser BP (2021) High-throughput screening identifies ascorbyl palmitate as a SIRT2 deacetylase and defatty-acylase inhibitor. ChemMedChem 16: 3484-3494.

(6) Bi D, Yang J, Hong JY, Parikh P, Hinds N, Infanti J, Lin H, Weiser BP (2020) Substrate-dependent modulation of SIRT2 by a fluorescent probe, 1-aminoanthracene. Biochemistry 59: 3869-3878.

 (7) Weiser BP (2020) Analysis of uracil DNA glycosylase (UNG2) stimulation by replication protein A (RPA) at ssDNA-dsDNA junctions. Biochimica et Biophysica Acta- Proteins and Proteomics 1868: 140347.

(8) Weiser BP, Rodriguez G, Cole PA, Stivers JT (2018) N-terminal domain of human uracil DNA glycosylase (hUNG2) promotes targeting to uracil sites adjacent to ssDNA-dsDNA junctions. Nucleic Acids Research 46: 7169-7178.

 (9) Weiser BP, Stivers JT, Cole PA (2017) Investigation of N-terminal phospho-regulation of uracil DNA glycosylase (UNG2) using protein semisynthesis. Biophysical Journal 113: 393-401.

 (10) Weiser BP, Eckenhoff RG (2015) Propofol inhibits SIRT2 deacetylase through a conformation-specific, allosteric site. Journal of Biological Chemistry 290: 8559-8568.

 (11) Weiser BP, Salari R, Eckenhoff RG, Brannigan G (2014) Computational investigation of cholesterol binding sites on mitochondrial VDAC. Journal of Physical Chemistry B 118: 9852-9860.